Purification and Characterization of Chitinase from the Nematode – Fungus Paecilomyces sp. P1
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منابع مشابه
Purification and characterization of chitinase from Streptomyces sp. M-20.
Chitinase (EC 3.2.1.14) was isolated from the culture filtrate of Streptomyces sp. M-20 and purified by ammonium sulfate precipitation, DEAE-cellulose ion-exchange chromatography, and Sephadex G-100 gel filtration. No exochitinase activity was found in the culture filtrate. The molecular mass of the purified chitinase was 20 kDa, estimated by a sodium dodecyl sulfate-polyacrylamide gel electrop...
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A chitinase was purified from the culture filtrate of nigeran-degrading Arthrobacter sp. NHB-10 by precipitation with ammonium sulfate and column chromatographies on DEAE-Sephadex A-50 and Superose 12. The final preparation was homogenous in polyacrylamide gel electrophoresis. The molecular weight of the purified enzyme was 30,000 and its isoelectric point was 6.8. The optimum pH and temperatur...
متن کاملPurification and characterization of chitinase from a new species strain Pseudomonas sp. TKU008.
The chitinase producing strain TKU008 was isolated from the soil in Taiwan, and it was identified as a new species of Pseudomonas. The culture condition suitable for production of chitinase was found to be shaken at 30 degrees C for 4 days in 100 mL of medium containing 1% shrimp and crab shell powder, 0.1 % K2HPO4 and 0.05% MgSO4 . 7 H2O (pH 7). The TKU008 chitinase was suppressed by the simul...
متن کاملChitinase from Enterobacter sp. NRG4: Its purification, characterization and reaction pattern
Enterobacter sp. NRG4 was shown to excrete chitinase into the culture supernatant when cultivated in medium containing chitin. A 60 kDa extracellular chitinase was purified to homogeneity and characterized. The enzyme hydrolyzed swollen chitin, colloidal chitin, regenerated chitin and glycol chitin but did not hydrolyze chitosan. The chitinase exhibited Km and Vmax values of 1.43 mg ml and 83.3...
متن کاملPurification and Characterization of an Antifungal Chitinase from Arabidopsis thaliana.
Plants exhibit an altered pattern of protein synthesis in response to pathogen invasion and abiotic stress. One of these ;pathogenesis-related' proteins has been identified as chitinase, which is capable of inhibiting fungal growth in vitro. This observation has led to the suggestion that the in vivo role of chitinases is to protect plants against fungal invasion. Here, we report the purificati...
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ژورنال
عنوان ژورنال: VNU Journal of Science: Natural Sciences and Technology
سال: 2019
ISSN: 2588-1140,2615-9317
DOI: 10.25073/2588-1140/vnunst.4851